First Meeting - May 19, 2014 - Yale West Campus (Orange, CT)
April 9, 2014
High-coverage high-throughput characterization of breast cancer cell line proteomes using 10-plexed TMT on a Tribrid Mass Spectrometer
- Wilhelm Haas, Ph.D.
- Sponsered by THERMO SCIENTIFIC
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Social hour begins at 5:30. Presentation begins at 6:30.
An ever growing amount of genomic and pharmacogenomic data on cancer tissue and cell lines reveals the enormous heterogeneity and complexity of the disease but it also holds the promise to inform new strategies for its targeted and patient-specific treatment. It is due to technical limitations that the “cancer”-proteome has yet remained largely untapped in these studies. However, we believe that this data has an immense potential to improve our understanding of the basic principles underlying cancer and to guide us to new approaches in treating the diseases as the proteome is a cell’s functional entity and virtually all targeted therapeutics in cancer treatment are targeting proteins.
In this presentation, we show that the use of TMT-10 reagents for quantitative proteomics on 32 breast cancer cell lines using a synchronous precursor selection supported MS3 method on the Thermo Scientific™ Orbitrap™ Fusion™ mass spectrometer allows us to overcome throughput limitations in quantitative proteomics by enabling the quantification of almost 8000 proteins in only 4.5 hours per cell line.
We believe that this technology puts proteomics in a position to be used side by side with genomics tools in studying complex diseases that require the analyses of a large number of samples.